產品簡介:
Bafilomycin A1 巴佛洛霉素A1
產品標簽
Bafilomycin A1巴佛洛霉素A1;Vacuolar H+ ATPase (V-ATPase) 液泡型ATP合成酶;Endosomal Acidification Inhibitor 胞內體酸化抑制劑;Autophagy Inhibitor 自吞噬;CAS NO:88899-55-2;
產品信息
產品名稱
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產品編號
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CAS NO.
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規格
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價格(元)
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Bafilomycin A1 巴佛洛霉素A1
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MZ8005-25UG
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88899-55-2
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25μg
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500
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Bafilomycin A1 巴佛洛霉素A1
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MZ8005-500UG
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88899-55-2
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500μg
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1450
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Bafilomycin A1 巴佛洛霉素A1
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MZ8005-1MG
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88899-55-2
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1mg
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2750
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產品描述
巴佛洛霉素A1(Bafilomycin A1),也稱為NSC 381866,來源于灰色鏈霉菌(Streptomyces griseus)的一種
大環內酯類抗生素。巴佛洛霉素A1是一種強效、選擇性的液泡型ATP合成酶(V-ATPase)抑制劑,在哺乳動物、植物或真菌細胞內阻斷這些質子泵活性,IC50在4-400nM范圍內。對大多數其他ATPase的抑制活性至少弱1000倍。Bafilomycin A1是一種知名的自吞噬晚期抑制劑,通過抑制自吞噬體和溶酶體之間的融合來阻止自體吞噬泡的成熟。Bafilomycin A1能防止巨噬細胞溶酶體內的膽固醇轉運,并且用來區分不同類型ATPase。Bafilomycin A1還能抑制巨吞噬和促進結腸癌細胞凋亡發生。
產品特性
1) CAS NO:88899-55-2
2) 化學名:(3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R, 5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11- tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one
3) 同義名:NSC 381866
4) 分子式:C35H58O9
5) 分子量:622.83
6) 純度:>95%(HPLC)
7) 外觀:黃色結晶粉末
8) 溶解性:溶于DMSO(0.1mg/ml),無水乙醇(0.1mg/ml),甲醇,幾乎不溶于水
9) 化學結構圖:
保存與運輸方法
保存:-20℃干燥保存,3年有效。
運輸:冰袋運輸。
注意事項
1) 關于化合物溶解性:產品特性內的“≥”表明溶于標示濃度,但飽和溶解度未知。不同批次化合物的溶解度會有差異。
2) 為了讓化合物更好的溶解,可通過37℃加熱或(和)超聲波水浴中震動片刻來處理。若實驗所需濃度過大甚至達產品溶解極限,請添加助溶劑助溶或自行調整濃度。
3) 為了您的安全和健康,請穿實驗服并戴一次性手套操作。
儲存液制備
質量
溶劑體積
濃度
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25μg
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500μg
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1mg
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1mM
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40.1394 μL
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0.8027 mL
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1.6056 mL
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5mM
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8.0279 μL
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0.1605 mL
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0.3211 mL
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【溫馨提示】:請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產品失效。儲備液置于-80°C保存,約6個月有效;-20°C保存,約1個月有效。溶液儲存的過程中避免強光直射。
使用方法【源自文獻,僅作參考】
文獻1,Yasuo Hayashi, et al.(2006) Effects of bafilomycin A1, a vacuolar type H+ ATPase inhibitor, on the thermosensitivity of a human pancreatic cancer cell line, International Journal of Hyperthermia, 22:4, 275-285
體外研究(細胞水平):
細胞類型(Cell Type):AsPC-1 cells
配制方法(Formulation):Bafilomycin A1 was dissolved in DMSO and then diluted to appropriate concentrations with the culture medium. The final concentration of DMSO in the medium was 1% or less.
實驗方法(Assay):The thermosensitizing effect of bafilomycin A1 and EIPA on AsPC-1 cells was quantified using a MTT colorimetric assay. 100μl cell suspension (1×105 cells/ml) was added to each well of a 96-well plate and incubated for 72 h at 37℃. The culture medium was then removed and 100 ml of pH 6.8 or 7.4 HEPES- buffered RPMI 1640 medium containing 1 mM bafilomycin A1 and/or 10 mM EIPA were added to each well. The plates were capped tightly and immersed in a 37C or 44C water bath for varying lengths of time. MTT assay was done to detect cell viability.
體內研究(動物模型):
動物模型(Animal Model):BALB/cA nude mice (Jcl-nu)
配制方法(Formulation):For in vivo studies, Bafilomycin A1 was dissolved in ethanol and then diluted to appropriate concentrations with PBS. The final concentration of ethanol in the medium was 1% or less.
實驗方法(Assay):AsPC-1 cells were injected s.c. into the right thigh of 6-week-old male mice. When tumors grew to ~150 mm3, they were divided randomly into eight experimental groups: a control group, a bafilomycin A1 (1.0 mg kg-1) treated group, a EIPA (3.0 mg kg-1 ) treated group, a bafilomycin A1 (1.0 mg kg-1) and EIPA (3.0 mg kg-1) treated group, a heated-only group, a heated group treated with bafilomycin A1 (1.0 mg kg-1), a heated group treated with EIPA (3.0 mg kg-1), a heated group treated with bafilomycin A1 (1.0 mg kg-1) and EIPA (3.0 mg kg-1). The non-heated groups received i.p. injections of 1.0mg kg-1 of bafilomycin A1 and/or 3.0 mg kg-1 of EIPA or PBS. The heated groups were injected IP with the drugs and tumours were heated beginning 60 min.
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— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作,主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本,以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。
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