產品簡介:
Chicago Sky Blue 6B, BS Grade芝加哥天藍6B(滂胺天藍)
產品信息
產品名稱
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產品編號
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規格
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價格(元)
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Chicago Sky Blue 6B, 90%芝加哥天藍6B(滂胺天藍)
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MS4073B-25G
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25g
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993
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Chicago Sky Blue 6B, 95%芝加哥天藍6B(滂胺天藍)
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MS4073C-1G
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1g
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963
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產品描述
芝加哥天藍6B(Chicago Sky Blue 6B, CSB6B),又稱為滂胺天藍(Pontamine sky blue),是一種染料和谷氨酸鹽衍生物,具廣泛的生物活性。芝加哥天藍6B(CSB6B)是囊泡谷氨酸轉運體(vGLUTs)的競爭性抑制劑,CSB6B(0.1 mM)抑制大鼠腦部突觸囊泡的谷氨酸攝?。↘i=0.19μM),但不抑制突觸體介導的谷氨酸攝取。CSB6B也是鈣/鈣調素依賴性蛋白激酶磷酸酶(CaMKP)和其核異構型CaMKP-N 的抑制劑(IC50s分別是4.1和1μM)。CSB6B(17μM)抑制鈉離子-?;悄懰峁厕D運多肽(NTCP, IC50= 7.1μM),降低HepaRG細胞感染乙型肝炎病毒(HBV)和丁型肝炎病毒(HDV)。腦室注射CSB6B(0.5μg)抑制小鼠炎性疼痛,減低乙酸誘導的扭動和福爾馬林II期的傷害行為,對自發運動無影響。CSB6B還能被用作復染劑,降低免疫熒光組織化學制備中的背景自發熒光。
保存與運輸方法
保存:室溫避光干燥保存,至少2年有效。
運輸:室溫運輸。
產品特性
化學名:6,6'-[(3,3-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4-amino-5-hydroxy-1,3- napthalenedisulphonic acid] tetrasodium salt
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同義名:CSB6B; Direct Blue 1; Pontamine sky blue; C.I. 24410;直接湖藍6B;滂胺天藍;芝加哥天藍6B;
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CAS NO:2610-05-1
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MDL NO:MFCD00004020
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分子式:C34H24N6Na4O16S4
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分子量:992.79g/mol
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純度:≥90%(MS4073B);≥95%(MS4073C)
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外觀:藍色/黑色固體
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溶解性:易溶于水(≥10mg/ml),微溶于溶纖素,不溶于其他有機溶劑
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化學結構式:
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配制儲存液
質量
溶劑體積
濃度
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1mg
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5mg
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10mg
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1mM
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1.0073 mL
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5.0363 mL
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10.0725 mL
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5mM
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0.2015 mL
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1.0073 mL
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2.0145 mL
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10mM
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0.1007 mL
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0.5036 mL
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1.0073 mL
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【溫馨提示】:請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產品失效。儲備液置于-80°C保存,約6個月有效;-20°C保存,約1個月有效。
使用方法【源自文獻,僅作參考】
文獻1,Yifa O, Weisinger K, Bassat E, Li H, Kain D, Barr H, Kozer N, Genzelinakh A, Rajchman D, Eigler T, Umansky KB, Lendengolts D, Brener O, Bursac N, Tzahor E. The small molecule Chicago Sky Blue promotes heart repair following myocardial infarction in mice. JCI Insight. 2019 Nov 14;4(22):e128025. doi: 10.1172/jci.insight.128025. PMID: 31723055; PMCID: PMC6948869.
體外研究(細胞水平):
細胞類型(Cell Type):Cardiac cells and Bone marrow cells
配制方法(Preparation):Chicago Sky Blue 6B (CSB) was dissolved in DDW to 10 μM for all in vitro cardiac cell culture experiments, and 50 μM for BMDM experiments.
實驗方法(Assay):Bone marrow cells were isolated and plated in macrophage differentiation medium. After 6 days of culture, the medium was aspirated and replaced with fresh macrophage differentiation medium with the addition of 10 ng/mL LPS. After 24 hours the LPS-containing medium was aspirated, cells were washed twice with PBS, andwere treated with 50 μM CSB in macrophage differentiation medium. Twenty-four hours later the cells were washed and RNA was purified.
體內研究:
動物模型(Animal Model):12-week-old Hsd:ICR female mice (Envigo RMS)
給藥劑量(Dosages):In vivo: CSB was diluted in PBS. Injection by i.m.: 50 μL of 100 μM stock solution, which is 0.005 μmoles (5 μg). Injections by i.v.: 100 μL of 100 μM stock solution, which is 0.01 μmoles (10 μg).
實驗方法(Assay):Mice were sacrificed 21 days after MI following 7 injections of CSB. The first injection was 50 μL of 100 μM CSB and was injected i.m. into the myocardium. Six additional injections were 100 μL of 100 μM CSB and were injected i.v. into the tail vein. The last i.v. injection was given 14 days after MI. PBS-treated mice were examined for comparison. A total of 6 CSB-treated and 5 PBS-treated mice were examined.
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文獻2,Goolam, M., Xypolita, ME., Costello, I. et al. The transcriptional repressor Blimp1/PRDM1 regulates the maternal decidual response in mice. Nat Commun 11, 2782 (2020). https://doi.org/10.1038/s41467-020-16603-z
體內研究:
動物模型(Animal Model):Female C57BL/6 mice (6–10 weeks of age)
藥物配制(Preparation):1% (w/v) Chicago Sky Blue 6B in PBS
實驗方法(Assay):To visualise implantation sites,mice were injected intravenously with 0.1ml of 1% (w/v) Chicago Sky Blue 6B in PBS under anaesthetic and sacrificed by CO2 asphyxiation 10min later. Individual decidua were removed from the uterus and shaken in 25μl of formamide for 2 days at room temperature (RT), protected from light to extract the dye. After clearing by centrifugation (16,000×g for 5min), dye content in formamide extracts was quantitated spectrophotometrically using an ND1000 Nanodrop at 618nm relative to a standard curve of Chicago Sky Blue dye dissolved in formamide.
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文獻3,Afshar P, Larijani LV, Rouhanizadeh H. A comparison of conventional rapid methods in diagnosis of superficial and cutaneous mycoses based on KOH, Chicago sky blue 6B and calcofluor white stains. Iran J Microbiol. 2018 Dec;10(6):433-440. PMID: 30873272; PMCID: PMC6414738.
體外研究(細胞水平):
細胞類型(Cell Type):Cardiac cells and Bone marrow cells
配制方法(Preparation):Chicago Sky Blue 6B (CSB) was dissolved in DDW to 10 μM for all in vitro cardiac cell culture experiments, and 50 μM for BMDM experiments.
實驗方法(Assay):Bone marrow cells were isolated and plated in macrophage differentiation medium. After 6 days of culture, the medium was aspirated and replaced with fresh macrophage differentiation medium with the addition of 10 ng/mL LPS. After 24 hours the LPS-containing medium was aspirated, cells were washed twice with PBS, andwere treated with 50 μM CSB in macrophage differentiation medium. Twenty-four hours later the cells were washed and RNA was purified.
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— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作,主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本,以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。
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